Fm4-64/ FM4-64 and RFP red channel were excited at 552 nm and the emission spectra were between 570 and 610 nm.

Fm4-64. Common dyes include calcofluor white DAPI DASPMI FM4-64 and DIOC6. GFP only transgenic seedlings were used as a control.

One way to visualize it in fluorescence microscopy is the use of styryl-based dyes like FM 4-64 or FM 5-95. In each such profile one can use interpolation to find the position of the maximum with subpixel precision. 19001 - AT - Aqua Longpass.

The cells were stained with FM4-64 for 3 min.

The scan speed was 400 Hz at a resolution of 1024 1024 pixels. However in this case the perpendicular intensity profile is maximal in the neighborhood of the CM see Fig. 19000 - AT UVDAPI Longpass. Fluorescence images of bacteria stained with FM4-64 also display a smeared view of the CM.